MP08-13 MICRORNA-29a REGULATES TGF-β1-INDUCED EXPRESSION OF COLLAGEN 1a1 IN HUMAN UROTHELIAL CELLS

You have accessJournal of UrologyBladder & Urethra: Anatomy, Physiology Pharmacology (MP08)1 Sep 2021MP08-13 MICRORNA-29a REGULATES TGF-β1-INDUCED EXPRESSION OF COLLAGEN 1a1 IN HUMAN UROTHELIAL CELLS Zunyi Wang, Jianghui Hou, and Dale Bjorling WangZunyi Wang More articles by this author , HouJianghui Hou BjorlingDale View All Author Informationhttps://doi.org/10.1097/JU.0000000000001981.13AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: microRNA-29 (miR-29) exerts potent anti-fibrotic effect in various organs suppressing mRNAs encoding many extracellular matrix molecules, including collagens. Urothelial cells line the lumen bladder respond stimuli producing releasing a variety biologically active molecules. Transforming growth factor- β1 (TGF-β1), profibrotic cytokine, TGF-β receptors 1 2 are present urothelial cells. Bladder inflammation increases expression these proteins, suggesting functional role TGF-β1 both physiological pathophysiological conditions bladder. In study, we examined regulatory miR-29a on TGF-β–induced collagen primary human METHODS: Primary (HUCs) were transfected with 40 nM mimics or an inhibitor function for 24 hours. The subsequently treated (1 ng/ml) Total RNA was isolated, abundance mRNA determined real-time PCR normalized message S26, constitutively expressed ribosomal protein same sample. Collagen content cultured medium using Sircol™ assay. Data as fold change relation values (set 1) control untreated samples. RESULTS: significantly increased (5.5 ± 0.4 vs 0.1, n = 6, p <0.01). Treatment mimic reduced (0.4 0.05, <0.001 group) attenuated TGF-β1-enhanced (3.1 0.3, TGF-β1-treated group). contrast, treatment (1.8 0.2, <0.01 but did not increase TGF-β1-induced responses (6.6 0.7, 0.19 Moreover, stimulated release into culture (1.7 0.1 <0.001). abolished (p <0.01), while enhanced <0.05), effects TGF-β1. CONCLUSIONS: This is first report that regulates attenuates These results strongly suggest miR-29 may contribute maintaining regulation fibrosis disorders. Source Funding: NIH DK118145 © 2021 American Urological Association Education Research, Inc.FiguresReferencesRelatedDetails Volume 206Issue Supplement 3September 2021Page: e154-e155 Advertisement Copyright Permissions© Inc.MetricsAuthor Information Expand Loading ...